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KMID : 1007520100190030821
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Food Science and Biotechnology
2010 Volume.19 No. 3 p.821 ~ p.826
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Anthocyanins in Cowpea [Vigna unguiculata (L.) Walp. ssp. unguiculata]
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Ha Tae-Joung
Lee Myoung-Hee
Jeong Yu-Na
Lee Jin-Hwan
Han Sang-Ik
Park Chang-Hwan
Pae Suk-Bok
Hwang Chung-Dong
Baek In-Youl
Park Keum-Yong
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Abstract
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The aim of this study was to isolate and identify the anthocyanins in the black seed coated cowpea [Vigna unguiculata (L.) Walp. ssp. unguiculata] using reverse phase C-18 open column chromatography and high performance liquid chromatography (HPLC) with diode array detection and electro spray ionization/mass spectrometry (DAD-ESI/MS) analysis, respectively. Anthocyanins were extracted from the coat of black cowpea with 1% trifluoroacetic acid (TFA) in methanol, isolated by RP-C- 18 column chromatography, and their structures elucidated by 1D and 2D nuclear magnetic resonance (NMR) spectroscopy. The isolated anthocyanins were characterized as delphinidin-3-O-glucoside (2) and cyanidin-3-Oglucoside (4). Furthermore, 5 minor anthocyanins were detected and identified as delphinidin-3-O-galactoside (1), cyanidin-3-O-galactoside (3), petunidin-3-O-glucoside (5), peonidin-3-O-glucoside (7), and malvidin-3-O-glucoside (8) based on the fragmentation patterns of HPLC-DADESI/ MS analysis.
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KEYWORD
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Vigna unguiculata, cowpea, anthocyanin, high performance liquid chromatography
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